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14 DiNicolantonioJ

Briefly, after dissection the brain tissue was incubated for 10 min at 37 C in PBS containing trypsinEDTA (0.025%/0.01% w/v) and then mechanically dissociated at room temperature with a fire-polished Pasteur pipette

Slowly push the plunger to inject the medicationtake your time here, about 5-10 seconds for 1 ml